Identification of evolutionary important residues by means of an entropy based analysis of MSAs.
Alignment file

Number of HSRPs

Add pseudo counts?

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Upload a multiple sequence alignment (MSA) file in FASTA format first. The MSA must contain at least 120 sequences for a sound computation of mutual dependencies. The first sequence in the MSA will be used for numbering in the results. Please note that files larger than 15 MB are not accepted.
Here you find an example MSA file.
High Scoring Residue Pairs (HSRPs) are used to determined conn(k) values. A HSPR value of 75 is a good starting point. Alternatively, a fraction of 2.5% of all residues works well for many proteins.
The addition of pseudo counts helps to correct for small frequency values.